XT-NoVo® & TimeSeal®
The XT-NoVo® and TimeSeal® platform is an advanced end-to-end solution for the sub-zero preservation and transport of biologics. XT-NoVo® is a DMSO-free formulation that uses synthetic, biomimetic antifreeze peptoids to prevent ice formation at –5°C, safely preserving cells, tissues, and engineered products for up to five days. When paired with the TimeSeal® smart transporter, featuring real-time monitoring and temperature resilience, the system enables scalable, reliable cold chain logistics for the on-demand delivery of high-value biological therapies.
Beyond Borders and Below Zero°
Living Cells & Tissues on Demand
The Problem
Racing the Clock
is the slim time window to keep primary tissues viable at 2-8°C
Cryopreservation cannot preserve tissue integrity
of enrolled patients never receive treatment due to manufacturing and cold-chain failure
of tumor biopsies fail quality standards due to substandard preservation
With XT-NoVo® Preservation, You Now Have Time.
Biopreservation solution
XT-NoVo®
XT-NoVo® is a fully defined sub-zero biopreservation solution for fresh, on‑demand biologics. It contains synthetic mimics of natural antifreeze proteins, supports reduced metabolic demand, and prevents ice formation at −5°C while retaining structural and functional integrity without DMSO or other cytotoxic cryoprotectants.
- Ice-free at -5° C
- DMSO-free and non-toxic
- Chemically-defined
- Cells, primary tissues, organoids and 3D constructs
Smart Sensored Transporter
TimeSeal®
A smart, lightweight, and easy-to-use transporter for cells, organoids, tissues, and organs. Smart sensor technology allows precise tracking and resilience to temperature fluctuations for up to five days.
- Customizable and modular design
- No external power or perfusion needed
- Smart sensor temperature tracking
- Static and box configuration
Benefits of XT-NoVo® & TimeSeal®
Enabling non-toxic, ice-free, sub-zero preservation for regenerative medicines.
Proprietary peptoid technology eliminates damaging ice phase transitions at sub-zero temperatures, preserving cellular membranes and spatial architecture.
Serum-free, chemically-defined solution containing biomimetic peptoid that mimics natural antifreeze proteins, without toxicity of traditional additives like DMSO.
Sub-zero storage extends preservation from the traditional 4–24 hour window to five days, slowing metabolism, reducing damage, and maintaining viability and functionality well beyond 4°C limits.
Validated across cell lines, primary tissues, engineered tissues, 3D constructs, and organoids.
XT-NoVo® and TimeSeal® extend the shelf life of sensitive cell based biologics from one to two days up to five days at sub-zero storage conditions. This simplifies logistics and enables a more cost effective cold chain.
Fields of Application
XT-NoVo® TimeSeal® has been validated across a wide range of biological materials.
Cell Lines
- HEK293
- Jurkat
- K562
- CHO
- TF-1
- HL-1
- HEPG-2
- HEPG-2 clusters
Primary Cells
- Leukopak, PBMCs, T-Cells
- iPSCs, iPSC-derived cardiomyocytes & islets
- BM-MSCs
- UC-MSCs
- β islets
- Organoids
Tissue/Organ-on-chip
- Heart
- Lung
- Liver
- Neurons
Primary Tissues
- Full-thickness skin graft
- Cartilage tissue
Proven Results
Functional recovery of heart grafts following 24 hours of ischemia
After 24 hours of ischemia, heart grafts perserved with XT-NoVo® and TimeSeal® at -5°C maintained functional survival during Langendorff perfusion and post-transplant, outperforming HTK preservation at 4°C, which failed to support graft survival.
XT-NoVo® preserves "test-ready" 3D tissue-on-chip models across routine QC readouts
Viability and functional performance of HEPG-2 spheroids and air–liquid interface (ALI) airway models were evaluated after preservation in XT-NoVo® at −5°C, compared with conventional hypothermic storage. XT-NoVo® preserves high viability across HEPG-2 spheroids and ALI airway models, with stronger functional output (increased albumin secretion) in spheroids and maintained key barrier architecture (i.e. tight junctions) in ALI models.
Human islets function preserved over five days
Human islet function was assessed after five days of preservation in XT-NoVo® at -5°C and compared with standard-of-care UW solution at 4°C. Islets preserved in XT-NoVo® exhibit robust glucose-stimulated C-peptide secretion (left) and stimulation index values (right) within the range of fresh islets, meeting regulatory acceptance criteria and outperforming conventional hypothermic storage.
First-ever shipping demonstration of sub-zero preserved tissues
“Even after a 5,500-mile cross-country round trip over 48 hours at -5°C, our highly complex organoids were completely non-frozen and fully recovered with perfect viability and function, exceeding our expectations. It marks a major advancement in regenerative medicine and fundamentally changes what’s possible for scalable, on-demand tissue regeneration. Exciting times!”
– Luis Alvarez, Ph.D. CEO of Theradaptive
Ready to Transform Your Biopreservation?
XT-NoVo® and TimeSeal® deliver ice-free, DMSO-free, sub-zero biopreservation of biologics, extending viability from hours to days.
